Fadraciclib – Pd-1-pd-l 1 Inhibitor 1

Interrogation of novel CDK2/9 inhibitor fadraciclib (CYC065) as a potential therapeutic approach for AML

Abstract
Over the last 50 years, there has been a steady improvement in the treatment outcome of acute myeloid leukemia (AML). However, median survival in the elderly is still poor due to intolerance to intensive chemotherapy and higher numbers of patients with adverse cytogenetics. Fadraciclib (CYC065), a novel cyclin-dependent kinase (CDK) 2/9 inhibitor, has preclinical efficacy in AML. In AML cell lines, myeloid cell leukemia 1 (MCL-1) was downregulated following treatment with fadraciclib, resulting in a rapid induction of apoptosis. In addition, RNA polymerase II (RNAPII)-driven transcription was suppressed, rendering a global gene suppression. Rapid induction of apoptosis was observed in primary AML cells after treatment with fadraciclib for 6–8 h. Twenty-four hours continuous treatment further increased efficacy of fadraciclib. Although preliminary results showed that AML cell lines harboring KMT2A rearrangement (KMT2A-r) are more sensitive to fadraciclib, we found that the drug can induce apoptosis and decrease MCL-1 expression in primary AML cells, regardless of KMT2A status. Importantly, the diversity of genetic mutations observed in primary AML patient samples was associated with variable response to fadraciclib, confirming the need for patient stratification to enable a more effective and personalized treatment approach. Synergistic activity was demonstrated when fadraciclib was combined with the BCL-2 inhibitor venetoclax, or the conventional chemotherapy agents, cytarabine, or azacitidine, with the combination of fadraciclib and azacitidine having the most favorable therapeutic window. In summary, these results highlight the potential of fadraciclib as a novel therapeutic approach for AML.

Introduction
Acute myeloid leukemia (AML) is one of the most common hematologic malignancies, characterized by clonal, proliferative, and abnormally or poorly differ- entiated myeloid cells inﬁltrating the bone marrow, blood or extramedullary tissues1–3. Although treat- ment has progressed, in some AML patients,Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. Ifmaterial is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.(midostaurin19, gilteritinib20), IDH1/2 inhibitors (ivosi- denib21, enasidenib22,23), BCL-2 inhibitor (veneto- clax24,25), and cyclin-dependent kinase (CDK) inhibitors (CDKi; alvocidib26–28, fadraciclib29), which cause cell cycle arrest and induce apoptosis.The second generation CDKi fadraciclib is an orally available 2,6,9-trisubstituted purine analog designed to selectively inhibit CDK2 and CDK929–31. Direct inhibition of CDK2 results in cell cycle arrest of abnormally pro- liferating cells32. The blockade of CDK9 halts RNA polymerase II (RNAPII) transcriptional activity conse- quently inducing apoptosis33. Furthermore, CDK9 inhi- bition downregulates the anti-apoptotic protein myeloid cell leukemia 1 (MCL-1)34. Fadraciclib has already entered clinical trials in advanced solid tumors (NCT02552953), chronic lymphocytic leukemia (CLL) (NCT03739554) and AML (NCT04017546).In this study, fadraciclib was assessed for efﬁcacy against AML cell lines and primary AML samples in vitro either as a single agent or in combination with venetoclax (VEN), cytarabine (AraC), or azacitidine (AZA).

Results
Fadraciclib induces apoptosis of AML cell lines and reduces metabolic activity confirming anti-proliferative effectIC50 of fadraciclib was established by resazurin reduction assay at 24, 48, and 72 h. The IC50 of fadra- ciclib was lower at 72 h, compared to 24 and 48 h, showing that longer exposure increased drug potency (Fig. 1A). At 72 h, the IC50 of fadraciclib in OCI-AML3, MOLM-13 and MV4–11 was approximately 0.44 ±0.01 µM, 0.25 ± 0.01 µM and 0.52 ± 0.01 µM, respectively. As compared to the IC50, which is related to anti- proliferative activity, higher fadraciclib doses were required to induce apoptosis in 50% of cells, represented by the percentage of annexin V-positive cells (%annex- inV). 0.75, 0.5, and 1 µM of fadraciclib was required to induce apoptosis in 50% of cells in OCI-AML3, MOLM-13 and MV4–11, respectively (Fig. 1B, C). This was conﬁrmed by a signiﬁcant concentration-dependent increase in the percentage of active caspase-3-positive cells (%active caspase-3; Fig. 1D). Cell cycle analysis at 72 h demonstrated a signiﬁcant increase in the percen- tage of sub G0 (%subG0) population at concentrations of0.75 μM, 0.5 μM, and 1 μM of fadraciclib for OCI-AML3, MOLM-13, and MV4–11, respectively, with no evidence of cell cycle arrest (Fig. 1E). However, at 4 h, G1 arrest occurred in OCI-AML3 and MV4–11, but not MOLM- 13 (Supplementary Fig. S1).Taken together, fadraciclib potently induces apoptosis, increases the subG0 population, and reduces proliferation as evidenced by reduced metabolic activity in AML cell lines.Fadraciclib downregulates MCL-1, resulting in rapid induction of apoptosis in AML cell linesAML cell lines were treated with 1 µM fadraciclib, for 4 and 24 h. An inhibitory effect on downstream targets was demonstrated by immunoblotting (Fig. 2A). Inhibition of CDK2 and CDK9 was evaluated by phosphorylation levels at serine 807/serine 811 of retinoblastoma (Rb) and serine 2 of RNAPII, respectively. Rb was not expressed in OCI-AML3.

In MOLM-13, densitometry conﬁrmed a 75% reduction in Rb levels by 4 h (p < 0.0001) and greater than 99% at 24 h, with a corresponding reduction in the phosphorylated form (p-Rb). In MV4–11, only a modest reduction in the levels of Rb and p-Rb at 4 h, with Rb level returning to baseline and a modest increase in p-Rb observed at 24 h (Fig. 2A, B). At 4 h, in all cell lines, the level of RNAPII decreased by approximately 60% with a corresponding decrease in phosphoryla- tion (Fig. 2A, C); with similar results at 24 h.A signiﬁcant downregulation of MCL-1 occurred at 4 h in all cell lines and was maintained or further decreased at 24 h (Fig. 2A, D). This induced apoptosis as indicated by the accumulation of cleaved poly (ADP-ribose) polymerase-1 (Fig. 2A, E). The function of CDK1 was not perturbed as observed by an unchanged level of threonine 320 phosphorylated protein phosphatase 1 alpha (Sup- plementary Fig. S2A, B).Owing to the impact of fadraciclib on MCL-1 levels, relevant signaling pathways, controlling MCL-1 were investigated. Threonine 180/tyrosine 182 phosphorylated p38 MAPK was markedly increased at 4 h in MOLM-13 (p < 0.0001) and MV4–11 (p < 0.001), and at 24 h in MOLM-13, but not in OCI-AML3 (Supplementary Fig. S2A, C). A decrease in threonine 202/tyrosine 204 phos- phorylated Erk1/2 was also observed at 4 and 24 h (Sup- plementary Fig. S2A, D). By contrast, serine 473 phosphorylated Akt (Supplementary Fig. S2A, E) and serine 9 phosphorylated GSK3β (Supplementary Fig. S2A, F) were not signiﬁcantly changed.These results imply that stress response and apoptosis are potently induced in MOLM-13 and MV4–1135–38, and the observed downregulation of MCL-1 was unlikely to be via the proteasome-dependent degradation pathway modulated by GSK3β39.Fadraciclib alters cell cycle regulatory gene expression to induce apoptosisOCI-AML3, MOLM-13 and MV4–11 were treated with 0.75, 0.5, and 1 µM of fadraciclib, respectively, for 4 and 24 h and the effect on cell cycle regulatory gene expression investigated. The heatmap depicts fold change of gene expression as compared with NDC (Fig. 2F). Several target genes were downregulated following fadraciclib treatment, including; CDK family, tran- scription factors, key protein phosphatases, CDKI,DNA damage response regulators, anti-apoptotic related, and pro-apoptotic related genes (Supplemen- tary Figs. S3–S5).In particular, at 4 h, the expression of CDKs, especially CDK9, the transcription factor E2F1, and MCL1, were suppressed. PPP1R10 was also signiﬁcantly down- regulated (Fig. 2F, G). Effects on gene expression were less marked at 24 h. Conversely, the DNA damage response regulator gene CDKN1A, encoding p21Cip1, was upregu- lated at 24 h, indicating the intrinsic apoptosis pathway40 was activated following fadraciclib treatment (Supple- mentary Fig. S4B).Fadraciclib induces apoptosis and decreases MCL-1 expression in primary AML cells, regardless of KMT2A-PTD statusPublished results indicate AML cell lines bearing KMT2A rearrangements (KMT2A-r) are sensitive to fadraciclib29. Five primary AML samples harboring KMT2A-partial tandem duplication (PTD) and ﬁve pri- mary AML samples with KMT2A wild-type (WT) were tested with fadraciclib as a single agent (Table. 1). All samples were treated with 0.5 and 1 µM fadraciclib for 24 h, then the drug was washed out and cells were cul- tured in fresh media for up to 72 h.At 24, 48, and 72 h time points, fadraciclib treatment resulted in an increase in apoptosis in both KMT2A- PTD and KMT2A WT patient samples (Fig. 3A, B). The proportion of apoptotic cells signiﬁcantly increased over time. Speciﬁcally, at 72 h, a concentration- dependent maximal increase in %annexinV was observed in KMT2A-PTD patient samples following treatment with fadraciclib at 0.5 µM (20.5 ± 9.6%, p = 0.6051) and 1 µM (79.5 ± 12.9%, p < 0.0001), and inKMT2A WT patient samples following treatment with fadraciclib at 0.5 µM (33 ± 14.6%, p = 0.624) and 1 µM (74 ± 26%, p < 0.0001) compared with NDC (Fig. 3A). Responses were similar in KMT2A-PTD and KMT2A WT patient samples.A concentration-dependent increase in %active caspase- 3 was observed in both KMT2A-PTD and KMT2A WT patient samples following fadraciclib treatment (Fig. 3B). However, due to genetic heterogeneity of AML samples (Table. 1), this difference did not reach statistical signiﬁcance.Treatment with fadraciclib resulted in a time- and concentration-dependent decrease in MCL-1 expres- sion in both KMT2A-PTD and WT AML samples. Speciﬁcally, at 72 h, a decrease in MCL-1 in KMT2A- PTD patient samples at 0.5 µM (42.8 ± 20.3%, p = 0.9997) and 1 µM (5.4 ± 3.2%, p = 0.1852), and inKMT2A WT patient samples at 0.5 µM (31.2 ± 21.2%, p= 0.9463) and 1 µM (4 ± 1%, p = 0.1913) following fadraciclib treatment compared with NDC (Fig. 3C).Pulsed dosing with fadraciclib in primary AML cells results in apoptosis and reduces cell viability, but is less effective than continuous dosingTo determine if pulsed dosing achieved an optimalresponse, primary AML cells were treated for up to 8 h with fadraciclib at a concentration of 1 μM. This resulted in a signiﬁcant increase in %annexinV at both 6 and 8 h (p = 0.023 and p = 0.0411, respectively) and %active caspase-3 at 6 h (p = 0.0351) as compared with NDC (Supplementary Fig. S6A, B). As fadraciclib exerts a rapid effect, we investigated whether a 6-h pulse was as effective as 24-h continuous treatment at inducing apoptosis. Fadraciclib showed a higher %active caspase-3 after con- tinuous treatment compared to the pulsed treatment (Supplementary Fig. S6C). MCL-1 levels were signiﬁcantly reduced after 24-h continuous treatment compared to both the NDC and the 6-h pulsed treatment (p = 0.0167 and p = 0.0304, respectively; Supplementary Fig. S6D).Increased apoptosis of primary human AML samples in combination studies of fadraciclib + VEN, fadraciclib + AraC, and fadraciclib + AZAFor combination studies, six primary AML samples were selected based on the presence of the most common favorable (e.g., NPM1) and unfavorable mutations (e.g., FLT3-internal tandem duplication (ITD) and KMT2A- PTD), in order to assess the efﬁcacy of fadraciclib and its synergistic activity when combined with VEN, AraC, or AZA, towards these mutations.Drug combination studies exploit opportunities for reduced drug resistance, decreased toxicity, and efﬁcacy improvement41. Based on phase 1 clinical trial data, the maximum clinically achievable concentration of fadraci- clib is 6–7 µM when administered as a single agent42. In this study, much lower fadraciclib concentrations of0.25 µM (FAD1) and 0.5 µM (FAD2) in combination with VEN, AraC or AZA were assessed for efﬁcacy against primary AML cells. The concentrations selected were based on previous studies: 0.025 µM (VEN1) and 0.5 µM43 (VEN2); 0.01 µM (AraC1) and 0.1 µM44 (AraC2); 0.5 µM45(AZA1) and 2 µM46 (AZA2), aiming to optimize results for both sensitive and more therapy-resistant patient samples. All drug concentrations were within clinically achievable concentrations (1 µM VEN47, 1 µM AraC48, and 3–11 µM AZA49). Apoptosis, active caspase-3, cell cycle analyses, and proliferation assays were performed at 72 h.In primary AML samples, %annexinV and %active caspase-3 (Supplementary Figs. S7A–C and S8A–C, respectively) were not signiﬁcantly different at low con- centrations, with any drug combination pair tested. At high concentrations, a modest increase in %annexinV was observed; 82.2 ± 16.9% in FAD2 + VEN2 combination (as compared with 55.7 ± 14% in FAD2, p = 0.1059; andFadraciclib alone and low-dose combination therapies have limited cytotoxicity on normal hematopoietic cellsFadraciclib alone and in combination with VEN, AraC and AZA was assessed in normal CD34 + hematopoietic samples to determine toxicity. Neither fadraciclib alone nor lower concentration drug combinations exerted a signiﬁcant increase in %annexinV, %active caspase-3 or % subG0 (Supplementary Figs. S7D–F, S8D–F, and S9D–F, respectively) as compared to NDC. However, as expected, an increase in %annexinV was observed at high con- centrations with a corresponding increase in %active caspase-3 (Supplementary Figs. S7D–F and S8D–F, respectively). Fadraciclib + AZA combination therapy has a therapeutic window in in vitro cell proliferation assaysFinally, cell proliferation assays using CellTrace Violet staining were performed. Representative histograms for AML and normal CD34 + samples are shown (Supple- mentary Fig. S10). When considering the combination therapies, none of the low concentration drug combi- nations resulted in a signiﬁcant anti-proliferative effect (increased percentage of undivided population, %Undi- vided %undivided) compared to either NDC or single drug therapies in either AML or normal CD34 + sam- ples (Fig. 4A–F).However, differences occurred with the high con- centration drug combinations. Speciﬁcally, FAD2 + VEN2, had signiﬁcant anti-proliferative effects when compared to VEN2, but might be explained by the effect of FAD2 (Fig. 4A). This combination had no anti- proliferative effect on normal CD34 + cells (Fig. 4D). Although FAD2 + AraC2, has signiﬁcantly increased anti-proliferative effect as compared to FAD2 in both AML and normal CD34 + samples, this is fully accounted for by the known cytotoxic effects of AraC (Fig. 4B, E). For FAD2 + AZA2, the anti-proliferative effect in AML samples was signiﬁcantly increased compared to both FAD2 and AZA2 (%Undivided % undivided of 68.7 ± 29.1% for FAD2 + AZA2 compared with 28.5 ± 10.2% in FAD2, p = 0.0004; and 33.5 ± 17%in AZA2, p = 0.0024), as well as NDC (Fig. 4C), indi- cating synergism of this combination with a combina- tion index (CI) of 0.75. No anti-proliferative effect was demonstrated for this combination on normal CD34 + cells (Fig. 4F).These ﬁndings indicate a therapeutic window and pro- vide evidence for further exploration of combination treatment with fadraciclib and AZA as a promising treatment approach. Discussion Our study evaluated the therapeutic potential of fadra- ciclib in AML. AML cell lines harboring various genetic lesions were tested, these include OCI-AML3 (carries NPM1 and DNMT3AR882C mutations), MOLM-13 (car- ries FLT3-ITD and KMT2A-MLLT3), and MV4–11 (car- ries FLT3-ITD and KMT2A-AFF1), aiming to encompass stratiﬁcation of major risk proﬁles according to the Eur- opean LeukemiaNet recommendations50. Results indicate MV4–11, harboring adverse prognosis molecular abnormalities, was less sensitive with the highest IC50 (Fig. 1A) and a higher dose of fadraciclib required to induce apoptosis (Fig. 1C–E).Inhibition of CDK2 and CDK9 was evaluated by phos- phorylation of Rb51,52 and RNAPII53,54. We found the effects of fadraciclib on Rb were cell line speciﬁc (Fig. 2A, B), whereas, the endogenous level of RNAPII was decreased in all cell lines (Fig. 2A, C). A transcriptional downregulation of RNAPII is the most plausible expla- nation for the global gene suppression observed (Fig. 2F and Supplementary Figs. S3–S5). This is consistent with a previous study stating that a downregulation of POLR2A encoding the major subunit of RNAPII was observed in leukemic blasts following treatment with alvocidib, which potently inhibits CDK955. A signiﬁcant decrease in short half-life MCL-1 at gene (Fig. 2G) and protein (Fig. 2A, D) levels highlights a potential target inhibited by fadraciclib. A rapid decrease in MCL-1 level perturbs a balance in the BCL-2 family, activating the intrinsic apoptosis path- way (Fig. 5B). AML cells are more dependent on MCL-1 than normal hematopoietic cells56, highlighting the potential of fadraciclib as a therapeutic approach for AML. Downregulation of E2F1 (Fig. 2G) may explain the G1 cell cycle arrest seen in OCI-AML3 and MV4–11 following fadraciclib treatment (Supplementary Fig. S1). Interestingly, Erk1/2 were suppressed in all cell lines tested (Supplementary Fig. S2A, D), which potentially promotes a reduction in MCL-1 stability and half-life57. In various cancer cell lines, siRNA-mediated PPP1R10 knockdown results in tumor suppressor PTEN release from nuclear sequestration58. Hence, the signiﬁcant decrease in PPP1R10 gene expression observed (Fig. 2G) potentially promotes an induction of apoptosis. Using leukemic blasts from adult patients with refractory AML in a phase 1 clinical trial (NCT00470197), treatment with the pan-CDKi alvocidib, resulted in a downregulation of various genes55. Among these, downregulation of POLR2A, encoding the major subunit of RNAPII, and E2F1 was seen. In addition, a decrease in phosphorylation of RNAPII and expression of MCL-1 was observed in leukemia blasts of some patients treated with alvocidib who achieved a CR59. Favorably, we found that the function of CDK1, which plays an important role in mitosis60,61, was not perturbed, suggesting better immune function with less impact on normal hematopoietic cells following fadraciclib treatment. Recently, a phase 1 clinical trial of fadraciclib in solid tumor patients was completed (NCT02552953)42. Dose- limiting toxicities were reversible and a biologically effective dose of 192 mg/m2/day, which corresponds to the drug concentration of 6–7 μM in vitro was established42.Over the past few years, many targeted therapies for patients with AML have emerged with efﬁcacy dependent on molecular subtype of AML62. This highlights the important of assessing the efﬁcacy of fadraciclib on spe- ciﬁc AML subtypes based on its mechanism of action of inhibiting CDK9. As part of the Super Elongation Com- plex (SEC), CDK9 is important for transcriptional elon- gation, in particular in KMT2A-r AML63, it is therefore rational to evaluate the efﬁcacy of fadraciclib in this AML subtype. Preliminary results indicate KMT2A-r AML cell lines are more sensitive to fadraciclib29. Patient samples, however, display a high diversity of genetic mutations(Table. 1). We therefore observed a more variable fadra- ciclib response with KMT2A-PTD status not being a predictor for better treatment response. This is consistent with previous results showing that the CDK9-speciﬁc inhibitor atuveciclib inhibits the proliferation of seven AML cell lines, regardless of KMT2A-r status64. In addi- tion, atuveciclib displayed potent in vitro activity in 80% of AML patient samples harboring KMT2A WT, includ- ing those with mutant NPM1 or FLT3-ITD.Considering the combination studies, primary AML samples were selected based on the most common favorable (e.g., NPM1) and unfavorable mutations (e.g., FLT3-ITD and KMT2A-PTD). Initially, we hypothesized that the treatment response would be relevant to the risk proﬁles of the individual genetic lesions in primary AML samples. However, we found that complexity of the molecular genetic lesions and karyotypes have more influence. The more complex the molecular landscape, the less efﬁcacious the combination therapy (Supple- mentary Table S4). The treatment response variability associated with the high diversity of genetic mutationsobserved reiterates the results of previous experiment showing the variable fadraciclib response, regardless of KMT2A-PTD status. Taken together, our results highlight the importance of genetic and molecular proﬁling prior to initiating therapy, to identify personalized therapeutic combinations most likely to beneﬁt individual patient. Regarding the 2017 ELN risk stratiﬁcation50, next gen- eration sequencing (NGS), exome sequencing and gen- ome wide assays65, are being developed to replace single gene assays. This will facilitate better prognostication and more personalized therapy in the future66. A short delay in commencing therapy in newly diagnosed AML had no effect on outcome after accounting for other prognostic covariates67, providing a potential window to perform NGS.As a global effect, an increase in cell death in primary human AML cells was shown for apoptosis, active caspase-3 assays, and cell cycle analysis when fadraciclib was combined with VEN, AraC, or AZA. In primary AML cells, combining the MCL-1 inhibitor S63845 with VEN results in greater efﬁcacy than either inhibitor alone, with a more potent activity against leukemic rather than nor- mal hematopoietic progenitors68. In an AML patient- derived xenograft mouse model bearing FLT3-ITD, the CDK9 inhibitor A-1592668 combined with VEN provided a signiﬁcant survival advantage over single treatments69. In a lymph node mimicking microenvironment, fadraci- clib in combination with VEN for 24 h efﬁciently induced apoptosis of primary CLL cells70, a disease where MCL-1 plays a role in disease progression and fludarabine resis- tance71. Ongoing phase 1 clinical trials are evaluating the safety and tolerability of fadraciclib in combination with VEN in patients with relapsed/refractory AML or mye- lodysplastic syndromes (MDS) (NCT04017546); and relapsed/refractory CLL (NCT03739554).When considering combining CDK9 inhibitors with AraC, a recent phase 2 study (NCT01349972), demon- strated a signiﬁcantly higher efﬁcacy of FLAM (alvocidib, cytarabine plus mitoxantrone) as compared with the standard “7 + 3” regimen in terms of a CR rate26,28. However, no increase in overall or event-free survival rates were observed. Potentially, this may be due to the higher toxicity of the combination regimen based on our preliminary data showing the impact of the fadraciclib + AraC on normal hematopoietic cells.In preliminary experiments, pre-treatment with alvoci- dib reduced the IC50 of AZA in MV4–11 cell line72. In the same manner seen in fadraciclib + AZA combination presented here, alvocidib + AZA also increased %active caspase-3 as compared with single treatments. In a MOLM-13 xenograft model, the combination showed greater tumor growth inhibition compared to single agent treatments. Our results, demonstrated a potential ther- apeutic window with the FAD2 + AZA2 combination, asmeasured by %Undivided %undivided of AML cells in cell proliferation assay (Fig. 4C). These interesting results identify an attractive therapeutic strategy warranting consideration of clinical development in AML.In summary, our studies indicate the preclinical efﬁcacy of the CDK2/9 inhibitor fadraciclib as a single agent or in conjunction with frontline AML chemotherapeutics, highlighting its potential as a novel therapeutic approach. Our work implicates the importance of molecular genetic proﬁling as a critical step prior to initiating therapy for AML, highlighting the need for a more personalized medicine approach to improve outcomes for patients.OCI-AML3, MOLM-13, and MV4–11 (DSMZ) werecultured at 37 °C in a 5% CO2 atmosphere in RPMI 1640 media (Sigma-Aldrich) with 20% or 10% fetal bovine serum (Gibco), supplemented with 1 mM glutamine and 1% penicillin-streptomycin (Invitrogen).Healthy donor and diagnostic primary AML samples were taken in accordance with the Declaration of Hel- sinki, and Ethics Committee approval (15/WS/0077). Demographic data, cytogenetic abnormalities and genetic lesions of AML patients are shown in Table. 1. Primary samples were thawed and cultured overnight in serum- free medium II (Stem Cell Technologies) supplemented with hIL-3, hIL-6, hSCF, and hFLT3L (all at 10 ng/mL) (Stem Cell Technologies).Ten micromolar stock solutions of fadraciclib (Cycla- cel), AZA (Stratech), and VEN (Stratech) were prepared in DMSO. 10 mM AraC (Sigma-Aldrich) was prepared in water. All stocks were stored at −20 °C and dilutions freshly prepared in cell culture media.Half-maximal inhibitory concentration (IC50) of AML cell lines treated with fadraciclib for up to 72 h was established using 7-Hydroxy-3H-phenoxazin-3-one-10- oxide sodium salt resazurin assay (Sigma-Aldrich) according to manufacturer’s instructions. IC50 were cal- culated using GraphPad Prism 8 software (GraphPad Software).RNA was extracted using RNA Easy Micro kits (Qiagen) and converted to cDNA using high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantita- tive reverse transcription polymerase chain reaction (qRT-PCR) was performed using Fluidigm Biomark technology and data were collected as per manufacturer’s instructions. Data was analyzed using the 2−ΔΔCTcompared to no drug control (NDC) as previously described73. Internal sample control was ensured by subtracting the average of six housekeeping genes ATP5F1B, B2M, CYC1, RNF20, TYW1, and UBE2D2 fromthe Ct value of each gene of interest. Mean and standard deviation of fold change in expression were calculated. For Primer sequences see Supplementary Table S1.In all, 1–5× 106 cells were harvested and washed twice in ice-cold PBS and pelleted at 300 × g for 5 min. Then, the pellets were lyzed in ice-cold solubilization buffer containing phosphatase and protease inhibitors at a con- centration of 1 × 106 cells per 10 μl solubilization buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Nonidet P40, 10% Glycerol, cOmplet ULTRA 1 tablet and PhosSTOP phosphatase inhibitor 1 tablet per 10 mL of solubilization buffer). This suspension was left on ice and vortexed every 15 min for 1 h, then centrifuged at 14,000 × g for 10 min to pellet all cell debris. The supernatant was transferred to a fresh eppendorf and stored at −80 o C until required.Protein concentration was quantiﬁed using the Quick Start Bradford Dye reagent and compared to a standard curve prepared using Quick StartTM BSA protein. The colorimetric protein assay was measured by visual absorbance on the Spectramax M5 plate reader at 595 nm at using SoftMax Pro software.Cell lysates were prepared and 30 µg of protein was resolved. Western blotting was performed using the NuPAGE electrophoresis system (Invitrogen) as per manufacturer’s instructions, and protein detection via Odyssey Fc Imaging System. Densitometry was performed using Image Studio Lite version 5.2. Antibodies and experimental conditions are outlined Fadraciclib in Supplementary Table S2.